FIG. 6.

ATP requirement for TFIIIC-dependent transcription. (A) Experimental scheme of ATP depletion for transcription. pCS6 chromatin templates (lanes 3 to 8) were subjected to gel filtration to remove ATP. For lanes 4 and 7, chromatin was replenished with ATP and creatine phosphate (CP) prior to the addition of TFIIIB, with or without TFIIIC, for transcription. NTPs, nucleoside triphosphates. (B) ATP dependence of SNR6 chromatin transcription. Transcription according to the scheme in panel A is shown in lanes 3 to 8. Lanes 1 and 2 show naked DNA transcription with or without TFIIIC, as indicated. Positions of the transcript seen as a 98-nt primer extension product and the recovery marker on the gel are indicated. (C) Transcript yields were normalized for sample recovery. The fold increase represents the ratio of transcripts generated with and without TFIIIC under otherwise identical conditions specified below the graph. Error bars indicate standard deviations of four independent experiments.