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. 2004 May;24(9):4075–4082. doi: 10.1128/MCB.24.9.4075-4082.2004

FIG. 1.

FIG. 1.

Targeted disruption of the SPI3 gene. (A) Exon 2 of the SPI3 gene was replaced with EGFP fused in frame with the SPI3 initiation codon. The pgk-loxPneo selection cassette was removed by crossing SPI3 heterozygotes with Cre-deleter mice. St, StuI; S, SmaI; X, XbaI; B, BamHI; Nh, NheI; N, NotI; WT, wild type; KO, knockout. (B) XbaI-cleaved DNA from wild-type cells (+/+) and recombinant cells (+/−) was hybridized to the 3′ probe, which distinguishes the wild-type allele (9.5 kb) from the targeted allele (7.5 kb). (C) To confirm correct integration of the 5′ arm, DNA from wild-type (+/+) and heterozygous (+/−) cells was subjected to PCR using primers 461 and 467, which amplify a 4-kb product from the wild-type allele, and primers 462 and 467, which amplify a 4-kb product from the targeted allele. (D) Wild-type (+/+), heterozygous (+/−), and knockout mice were typed by PCR of tail DNA with primers 152, 688, and 462 simultaneously.