FIG. 5.
Response to C. albicans in vitro and in vivo. (A) Wild-type (open bar) and SPI3-deficient (grey bar) mice were injected intraperitoneally with culture filtrate from C. albicans. Neutrophils were isolated from the peritoneal cavity 4 h later and tested for the ability to kill either the blastoconidial or hyphal form of C. albicans. Results represent the mean (± one standard error of the mean) (n = 6 for each genotype) proportion of C. albicans surviving after being incubated with peritoneal cells compared to that of C. albicans incubated in the absence of cells. (B) Wild-type (open symbols) and SPI3-deficient (closed symbols) mice were injected intravenously with 2.5 × 106 CFU of C. albicans and killed 3 weeks later, and the yeast load in the liver, lungs, spleen, and kidneys was determined. Yeast load is expressed as CFU/mg of tissue, each symbol represents data from one mouse, and the bar represents the mean for each group (n = 6 for each genotype).