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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Mol Psychiatry. 2013 Sep 3;19(7):801–810. doi: 10.1038/mp.2013.104

Figure 2. Chronic treatment with nicotine and/or 24hWD significantly increases neuregulin 3, but not neuregulin 1, in the hippocampus.

Figure 2

Animals were treated in vivo with either saline, nicotine (18 mg/kg/day), or undergoing 24h withdrawal (WD) from nicotine. Hippocampal tissues from treated animals were used in qPCR or Western blot experiments to evaluate mRNA or protein levels. As shown, chronic treatment with nicotine or 24hWD does not alter neuregulin 1 (NRG1) (A) mRNA or (D) protein levels in the hippocampus. In contrast, mRNA (B) and protein (E) levels of ErbB4 are increased following chronic treatment with nicotine and/or during 24hWD. Additionally, mRNA levels of the CREB target neuregulin 3 are increased following chronic nicotine treatment and 24hWD (C). Furthermore, protein levels of NRG3 (F) are increased following 24hWD in wildtype (WT), but not CREB mutant, mice. Representative blots are shown below each panel. *, p<0.05; **, p<0.01. A–C: N=6–11; D–F: 6–10.