Fig. 3.

(A–D) Representative images of immunocytochemistry show that differentiated neural cells derived from both mutant LRRK2^G2019S/WT and gene-corrected LRRK2^WT/WT iPSCs show equivalent expression of general neuronal markers (synapsin and alpha-synuclein) as well as robust expression of dopaminergic markers (TH & VMAT2). While GABA-containing neurons were also seen, astroglia (GFAP) were not (data not shown). Also note that our previous work provided compelling electrophysiological evidence that our protocol produces a true neuronal phenotype (Cooper et al., 2012).