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Relative level of LILRA6/B3 mRNA expression correlates with the CNV genotype. Total RNA was isolated from PBMC of healthy donors and the corresponding cDNA was used in SYBR Green qPCR. The PCR_mix is an equimolar mixture of LILRB3 and LILRA6 PCR fragments. Genomic DNA from an individual with two copies of LILRA6 was used as a reference control (gDNA, LILRA6/LILRB3=1). All reactions were performed in triplicate.
