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. 2004 May;24(9):4065–4074. doi: 10.1128/MCB.24.9.4065-4074.2004

FIG. 3.

FIG. 3.

Protein kinase domain of MOK was essential for the binding of Cdc37. Various MOK deletion mutants with a FLAG tag were expressed in COS7 cells and immunoprecipitated. (A) Amounts of MOK and its mutants in the immunoprecipitates were examined by Western blotting. Bands corresponding to deletion mutant proteins are indicated by asterisks [except MOK(N107) (see the text)]. Lane 1, control (not transfected); lane 2, MOK(WT); lane 3, MOK(N107); lane 4, MOK(N178); lane 5, MOK(N285); lane 6, MOK(D8-078); lane 7, MOK(D8-195); lane 8, MOK(D8-309). (B) Associations of endogenous Cdc37 with the same set of MOK deletion mutants as in panel A were examined by coimmunoprecipitation experiments and revealed by Western blotting with anti-Cdc37 antibody. Lane marks are the same as those for panel A. The positions of Cdc37, immunoglobulin heavy-chain (HC), immunoglobulin light-chain (LC), and molecular weight markers are indicated. (C) The relationship between structures and the Cdc37 binding abilities of MOK deletion mutants is schematically illustrated. ++, binds strongly; +, binds well; −, no binding.