Table 4.
Summary of the analysis of spine turnover and the measurement of spine head areas in hippocampal neurons in culture.
| Conditions | Number of dendrites | % of dendrites showing spines that disappear | % of dendrites showing de novo spines |
|---|---|---|---|
| Control | 22 | 45.5 | 54.5 |
| PI3K | 23 | 17.4 | 82.6 |
| Mean head area (μm2) 19DIV* | Mean area (μm2) of persistent spines (48 h) | Mean area (μm2) of de novo spines (48 h) | |
| Control | 0.74 ± 0.04 | 0.66 ± 0.04 | 0.63 ± 0.04 |
| PI3K | 0.73 ± 0.04 | 0.56 ± 0.03 | 0.36 ± 0.02 |
Time lapse analysis of spine turnover in control conditions (11 cells in one culture) and after PI3K overactivation (10 cells in one culture). *19DIV correspond to control conditions. PTD4-PI3KAc was administered to the culture medium just after the start of time lapse recordings. Notice that in Figure 8 both controls have been averaged together (white bar; 0.73 ± 0.03 μm2).