Figure 1.

Sequences and Expression Characteristics of PAM68 Proteins.

(A) Phylogenetic analysis of PAM68 and PAM68L sequences from cyanobacteria (shaded in blue), green algae (dark green), heterokont algae (brown), and land plants (light green). Full species names and accession numbers are provided in Methods; an alignment of the sequences used for tree construction is shown in Supplemental Figure 4 online. Branch lengths reflect the estimated number of substitutions per 100 sites.

(B) Expression characteristics of PAM68 and PAM68L. Expression profiling of the genes coding for PAM68 and PAM68L revealed tight coregulation with SIG6, PDE191, CRR42, and CRR6, respectively. Additional correlations with expression of genes for the envelope proteins TIC110 and TOC33, the photosynthetic electron transport components PSBX, PSAF, and plastocyanin (PETE1), and the nonchloroplast protein CRY2 were hierarchically clustered based on profile similarities using the single linkage method (gene coexpression database ATTEDII; http://atted.jp/). The degree of coexpression (COR) is measured by Pearson's correlation (r). Low distance (height) values (1-r) indicate high expression similarities.