Figure 4.

Absence of PAM68L or CRR4 Has Similar Effects on the Composition and Assembly of NDH Subcomplexes.

(A) and (B) Immunoblot analysis of thylakoid proteins from the wild type, the pam68l-1 mutant, and other mutants that are known to affect the composition and/or assembly of the NDH-C. Thylakoid proteins (5 µg chlorophyll) from the wild type (Col-0) and the different mutants were loaded, together with a dilution series of the wild-type sample as indicated.

(C) Schematic overview of the subcomplex composition of NDH in the chloroplasts of land plants (Ifuku et al., 2011). For designations of subcomplexes, see main text. The marker subunits for individual subcomplexes that were analyzed here (and in Figure 7) are indicated in bold.

(D) Hierarchical clustering of the NDH subunit patterns found in the different genotypes analyzed in (A) and (B). The original average values determined from immunoblot analyses (representatives of which are shown in [A] and [B]) are provided in Supplemental Table 1 online. The four classes discussed in the main text are indicated. WT, the wild type.