Figure 3.

Neighboring Cells Can Rescue Tracheary Element Lignification in Arabidopsis Roots.

(A) Wild-type (WT) root stained with basic fuchsin shows well lignified tracheary elements (arrow).

(B) Pro35S:miRNA CCR1-expressing roots still develop tracheary elements (arrows).

(C) In Pro35S:miRNA CCR1, no fluorescence above background is observed in the areas of the tracheary elements (arrow).

(D) Tracheary elements (arrow) are the only cell types labeled in the late elongation zone by expression of ProCESA7:GFP.

(E) If downregulation of monolignol biosynthesis is restricted to tracheary elements by expression of tracheary element–specific ProCESA7:miRNA CCR1, basic fuchsin stain indicates lignification similar to the wild type.

Bars = 10 μm.