Figure 3.

Expression of MYB14, MYB15, STSs, and Stilbene Accumulation during Grape Berry Development of V. vinifera cv Pinot Noir.

(A) Columns represent MYB and STS transcript profiles in flower/skins.

(B) Stilbene accumulation in flower/skins.

(C) MYB and STS transcript profiles in seeds.

(D) Stilbene accumulation in seeds.

Data points are given as weeks from the onset of ripening (véraison; labeled with an arrow), with expression values as means of three replicate PCRs (n = 6) and error bars indicating se. Note that from 6 weeks before véraison (-6), berry skin has been separated from seeds. Flowering occurred 8 weeks before véraison. Accumulation of stilbenes was measured as methanol extracts of developing grape tissues using reverse-phase HPLC analysis. (B) and (D) show relative stilbene contents of trans-piceid in developing berries and in seeds as the mean of three different experiments, with error bars indicating se. Note that each time point during developmental series is a pool of >100 berries collected from ∼20 plants growing as described in Methods. FW, fresh weight.