Figure 6.

GFP-PTS1 Localization Reveals Import and Morphology Defects in lon2-2 and Normal Appearance of atg7-3 Peroxisomes.

Cotyledon mesophyll and epidermal cells in 4- and 8-d-old wild-type (Wt) (A), lon2-2 (B), and atg7-3 (C) seedlings expressing 35S:GFP-PTS1 were imaged for GFP fluorescence (green) using confocal microscopy. Mesophyll cells also were imaged for chlorophyll autofluorescence (magenta) to visualize chloroplasts. Epidermal cells from 8-d-old seedlings were imaged at both normal (left panels) and heightened (right panels) gain settings to allow clear visualization of the GFP-PTS1 import defect of the lon2-2 mutant. Bar = 20 μm.