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. 2013 Oct 25;25(10):3858–3870. doi: 10.1105/tpc.113.114421

Figure 2.

Figure 2.

Concentrations of IAA Metabolites in IAA-Overproducing Arabidopsis Mutant Lines.

Free IAA, the catabolite oxIAA, and the conjugates IAA-Asp and IAA-Glu were quantified in root tissues from 10-DAG wild-type Arabidopsis Col-0 and IAA-overproducing lines (35S:YUC1, sur1-3, and sur2-1).

(A) Logarithmic concentration (pmol g−1 fresh weight) of IAA, oxIAA, IAA-Asp, and IAA-Glu in Arabidopsis Col-0 (black bars) and the mutant lines 35S:YUC1, sur1-3, and sur2-1 (dark to light gray bars, respectively). Error bars indicate sd (n = 3). Asterisks indicate statistically significant differences in the mutant lines (35S:YUC1, sur1-3, and sur2-1) versus the wild type (Col-0) in an ANOVA analysis (one, two, and three asterisks correspond to 0.05 > P > 0.01, 0.01 > P > 0.001, and P < 0.001, respectively). FW, Fresh weight.

(B) Logarithmic ratio between the concentration of IAA metabolites and free IAA (diamonds, oxIAA/IAA; squares, IAA-Asp/IAA; triangles, IAA-Glu/IAA) in the different Arabidopsis lines.