Figure 4.

Zymogram Analysis of Starch-Metabolizing Enzymes from Wild-Type and Class A Mutants.

Total protein of wild-type (WT) and class A mutant strains (21’C6, 80D5, 81G3, 91D6, 91G1, 99A7, 107D8, 118H9, 123B3, 134A4, 153H12, 174B6, 174H3, and 175E12) were separated by native PAGE, followed by transfer of proteins to a native PAGE containing 0.6% (p/v) amylopectin. The native gels were then incubated with (A) or without (B) 10 mM MgCl₂. A cation-dependent activity enzyme is witnessed after iodine staining in the wild type’s crude extract and disappears in all class A mutants (black arrow).