Figure 2.
Analyses of Arabidopsis DEX-Inducible At-CPP1 RNAi Lines.
(A) Phenotypes of DEX-inducible At-CPP1 RNAi seedlings. The RNAi seedlings (line #1) were germinated and grown under low light (20 μmol m−2 s−1) or high light (200 μmol m−2 s−1) on medium containing ethanol (−DEX) or DEX.
(B) NBT staining to visualize superoxide production in the RNAi seedlings (lines #1 and #4) grown under low light (20 μmol m−2 s−1). The cotyledons of the seedlings grown on medium with DEX exhibited a strong blue color indicating excessive production of superoxide.
(C) Leaf yellowing following DEX treatment. RNAi seedlings (line #1) were grown for 2 weeks on soil and then sprayed with ethanol (−DEX) or 30 μM DEX for 10 d. Leaf morphology of the RNAi plants following 10 d of ethanol or DEX spraying is also shown.
(D) Real-time quantitative RT-PCR analysis with the RNAi seedlings (line #1) confirmed silencing of At-CPP1 upon spraying with DEX (0, 4, and 8 d). Data points represent means ± sd of three experiments. Asterisks denote the statistical significance of the differences between the samples. **P ≤ 0.01.