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Presence of multiple biotinylated proteins in O. sagaranesis. Biotinylated proteins in the cell extracts of O. sagaranensis were partially purified with TetraLink tetrameric avidin resin as described in Materials and Methods. The partially purified extract was subjected to SDS-PAGE and detected with streptavidin-HRP conjugate. The arrowheads indicate the positions of three biotinylated proteins with molecular masses of approximately 130, 120, and 70 kDa.
