Figure 2.

Differential production of dendritic-like cells in stromal co-cultures established with different progenitors. Co-cultures were established over 5G3 as in Figure 1. Cell production was assessed after 14, 21, 28, and 35 days using antibody staining and multicolor flow cytometry. Cell production was assessed in terms of proportion of each subset, and number of cells of each type produced. Graphs show mean ± SE for triplicate co-cultures. (A) Production of CD11b⁻CD11c^−/lo and CD11b⁺CD11c⁺ cells. (B) Production of CD11b⁺CD11c⁺ cells differing in expression of MHC-II. All data for days 21, 28, 35, but only some 14 days data, are significantly different (p ≤ 0.05).