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. 2014 Jan 2;4:501. doi: 10.3389/fimmu.2013.00501

Figure 7.

Figure 7

M-CSFR inhibitor selectively inhibits development of cDC-like cells but not L-DC. Lin− BM from C57BL/6J mice was co-cultured with 5G3 stroma in the absence or presence of 10 nM GW2580, an inhibitor of M-CSFR, added again to cultures at medium change every 3–4 days. (A) Cell production was analyzed at 14, 21, and 28 days, by staining cells for CD11c, CD11b, MHC-II, 4-1BBL, F4/80, and CD8α, followed by flow cytometric analysis. Propidium iodide staining was used to delineate live (PI−) cells, which were then gated on CD11c and CD11b using isotype controls to set gates. L-DC were then distinguished from cDC-like cells on the basis of expression of 4-1BBL, F4/80, and the absence of MHC-II expression. (B) Co-cultures were analyzed over time for production of CD11b+ cells differing in expression of CD11c, and for production of CD11c+CD11b+ cells differing in expression of MHC-II. Proportion of each subset is shown as mean ± SE for triplicate co-cultures. All data are significantly different (p ≤ 0.05).