TABLE 1.

Primers used for reporter gene fusions and mutagenesis

Primer	DNA sequence (5′ → 3′) with incorporated restriction site underlined	Application	Accession no.
spaP5′	ATGGATCCAGCAAAAATTGACAAATCAAATC	spaP promoter	X17390
spaP3′	TTCTGCAGCATAAATCCTCCAAATCTGAATAAATC
gbpB5′	TAGGATCCTATGAAAGGCGATGTTAAAGC	gbpB promoter	AY046410
gbpB3′	TTCTGCAGATATAACTCCTTTTTCGATAAGAC
gtfB5′	TAGGATCCGACAATTGTGGTGGGTAC	gtfB promoter	M17361
gtfB3′	TACTGCAGCTTGTCCATTAGGAACCTCC
gtfC5′	ATGGATCCGATGCTAACTCTGGAGAACG	gtfC promoter	M17361
gtfC3′	ATCTGCAGTCCAAAAATAGTTAGAGTTAGTG
ftf5′	GGGGATCCTAGCTAGTGGACAGACTCTG	ftf promoter	M18954
ftf3′	AACTGCAGTTTCCATTAGCAAACCTCC
fruA5′	TTGGATCCGAGCATTAATGACATCTGTCATATTAAG	fruA promoter	U78296
fruA3′	ATCTGCAGTTTTCAAATTTATGAAACTGACAAACTC
up5′	CAACATCAAGCGAAGCCTCGAC	5′ region of smu486	AE014133
up3′	TTGAGCTCCATATACGTTCTACCTCCACC
down5′	TTAAGCTTCCTGTCATTGAAGCAGGTGC	3′ region of smu487	AE014133
down3′	GAATCAGGCATATAACCCGTATC
pluxS5′	AAGGATCCGCATAACCAATAACCAAACC	luxS promoter	AE014133
pluxS3′	TCCTGCAGAAAGCTTTCAACAGTAACTTC