Comparison of H2-facilitated anaerobic growth of S. Typhimurium strains. Anaerobic WT/JSG210 (represented as circles), RLK7/ΔhycC (represented as diamonds) and RLK6/ΔgudT (represented as triangles) in minimal medium with 0.4% d-glucarate. Solid lines indicate growth with added H2 (20% v/v) and dashed lines indicate growth without added H2. The standard deviation was less than 4% of the mean in every case, so that the added H2 condition is significantly greater than without H2 for each individual strain for all points at 4 h and greater at p ≤ 0.05 by Student's t-test. Without H2, there was also a significant difference in JSG210 and the RLK7 mutant at 6 and 8 h points (p ≤ 0.05 by Student's t-test), while with H2, the WT was significantly greater than strain RLK6 only.