Table 2.
Primers used in this study.
| primer | primer sequence (5′ → 3′) | application |
|---|---|---|
| gudT del-F | TGAGCGTAGCTAACGCGAAATTTCAGGA- GTGCAACATGTGTAGGCTGGAGCTGCTTC |
gudT deletion |
| gudT del-R | CCTTCATGTCCGTAATAACGGGACTGGAT- TGCGTTGTCACATATGAATATCCTCCTTA |
gudT deletion |
| gudT-check-F | GTTTGCTTGCGTTGCCAGTA | gudT-deletion confirmation |
| gudT-check-R | GTTCACAGACCGGCACATTA | gudT-deletion confirmation |
| gudT comp-F | GTCCTAGATCTTATGAGTTCATTAAGTCAC | gudT complementation |
| gudT comp-R | CTGGTGTCGACTCATGATAATTGTCCTGC | gudT complementation |
| hycC del-F | CTTGTTTCAGCAGGCTCAGAGTGGGGA- TGCATATGTGTAGGCTGGAGCTGCTTC |
hycC deletion |
| hycC del-R | GCGCCTGAATTAACGGATAAAACAC- ACTCATTTCATATGAATATCCTCCTTA |
hycC deletion |
| hycC-check-F | GTGAGCTGACGTTTAATACCGA | hycC deletion confirmation |
| hycC-check-R | CGACCGAGCAGTTTGATAATGT | hycC deletion confirmation |