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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: FEBS J. 2013 Oct 8;280(23):10.1111/febs.12529. doi: 10.1111/febs.12529

Figure 1. GPR56 is transiently expressed in the early differentiation phase of mouse myoblasts.

Figure 1

A. Phase images of mouse myoblasts induced to differentiate over the course of 6 days (D0 – D6) illustrating the degree of myotube formation. B. Upregulation of GPR56 mRNA expression at D1 by qRT-PCR in primary mouse myoblasts which then rapidly decreases. C. Protein expression of GPR56, MyoD, myogenin, and α/β-tubulin (loading control) in myoblasts at D0 to D6, as assessed by Western blot. GPR56 protein expression peaks at D1 and rapidly decreases by D3, where little expression remains. D–E. GPR56 (green) and caveolin-1 (red) staining in differentiating primary mouse myoblasts at D1. DAPI (blue) was used to stain nuclei. Arrows point to GPR56+ cells that are positioned closely and elongated, suggesting that the cells are readying for fusion or fusing. Scale bar = 50 µm.