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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: FEBS J. 2013 Oct 8;280(23):10.1111/febs.12529. doi: 10.1111/febs.12529

Figure 5. Loss of GPR56 affects the expression of myogenic transcription factors during regeneration but does not affect myofiber size.

Figure 5

A. H&E staining of GPR56 WT and KO gastrocnemius muscle at days 4, 6, and 18 after cardiotoxin injury. KO morphology and timing does not look different from WT. B. mRNA expression of GPR56 by RT-qPCR shows transient upregulation of GPR56 during regeneration. C. Myofiber diameter in cardiotoxin-injured WT and GPR56 KO gastrocnemius muscle shows no difference in diameter between WT and KO. D. mRNA expression by RT-qPCR of various genes in WT (black diamond) and GPR56 KO (gray circle) cardiotoxin-injured muscle. Myf5, MyoD, and myogenin are delayed in expression in KO muscle. * p<0.05, n = 3. E. Sample Western blots of myosin heavy chain protein expression. F. Quantification of the amount of MHC I, IIA, or IIB protein expression by Western blot in WT and KO gastrocnemius muscle in mice of various ages shows no difference in the amount of MHC isoforms between WT and KO. G. Quantification of the % of positive MHC I, IIA, or IIB fiber types in KO versus WT muscles, based on immunofluorescence staining in 4 littermate pairs.