TABLE 1.
Primers
| Primer nameand description | Primer sequencea (5′ to 3′) |
|---|---|
| Forward PCR primers | |
| JER074 wildtype | GGGAATTCATG CAATCCATTAACTTCCGT |
| JER075 Δ(2-17) | GGGAATTCATG AACAATGTTGAAGCCGG |
| JER076 Δ(2-35) | GGGAATTCATG GCAGTAATTGTCAGCAA |
| JER077 Δ(2-53) | GGGAATTCATG GCTGAATTTGCATCCCTG |
| Reverse PCR primers | |
| JER070 Δ(19-73) | GGGAAGCTTTTA GTTGAGCACTTCAGAAA |
| JER071 Δ(37-73) | GGGAAGCTTTTA TGCTGGCTCACGGCCTC |
| JER072 Δ(55-73) | GGGAAGCTTTTA AGCATCCAGCGCCGCTTT |
| JER073 wildtype | GGGAAGCTTTTA TCGGTTAACCAGTTCCTT |
| Sequencing primers | |
| JAS088F | CCATAAGATTAGCGGATCCTA |
| JAS090R | AGGTGGGACCACCGCGCTACTGC |
a
All primers are written 5′ to 3′. Primer encoded HindIII (AAGCTT) and EcoRI (GAATTC) sites are underlined. Primer encoded start codons (ATG) are italicized. Similarly, the reverse complements of the TAA ochre codons (TTA) are italicized.