TABLE 3.
Repressor activities of Phd deletions
| Relevant genotypea | β-Galactosidase sp actb | Fold repressionc | Repressor activity |
|---|---|---|---|
| PBAD (no insert) | 5,235 | 1.00 | No |
| PBAD-phd+ | 262 | 19.99 | Yes |
| PBAD-phdΔ(2-17) | 4,672 | 1.12 | No |
| PBAD-phdΔ(2-35) | 4,665 | 1.12 | No |
| PBAD-phdΔ(2-53) | 4,564 | 1.15 | No |
| PBAD-phdΔ(19-73) | 4,625 | 1.13 | No |
| PBAD-phdΔ(37-73) | 4,307 | 1.21 | No |
| PBAD-phdΔ(55-73) | 1,301 | 4.02 | Partial |
a
The indicated constructs were introduced into BR7024 and tested for their ability to repress a lacZ reporter fused to the P1 plasmid addiction promoter.
b
β-Galactosidase specific activity is the average from three separate experiments. The standard deviations were less than 10% of the means.
c
Fold repression is the ratio of the β-galactosidase specific activity of the unrepressed control strain (no insert) divided by the β-galactosidase specific activity of the experimental strain.