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. 2013 Dec 2;11(12):4834–4857. doi: 10.3390/md11124834

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Optimization of growth conditions for champacyclin (1a) biosynthesis of strain C42. In each experiment base parameters were maintained except for one parameter, which was variable in the experiment: 100 mL culture volume, actinomycetes medium except in the salt experiments GYM medium was used, 1.5% salt (NaCl), six days incubation time, 28 °C incubation temperature, 125 rpm shaking, and methanolic extracts. The results are comparable within each experiment series. Amino acids (1 mg/mL supplement): no = no supplementation, LIFV = _(L)leucine (L), _(L)isoleucine (I), _(L)phenylalanine (F) and _(L)valine (V). Temp. = Temperature. Media abbreviations see text.