Figure 1.

Modulation of serum sex steroid levels by dietary intake of EE₂ exposure. 17β-estradiol (E₂) (a), testosterone (T) (b) and 11-ketotestosterone (11KT) (c) serum levels were determined in gilthead seabream specimens in the pre-spermatogenesis (pre-SG) stage after the dietary intake of 0 (control) and 5 µg EE₂/g food during 7 and 28 days. The serum sex steroid levels (ng/mL) from five to six fish/group were analyzed by ELISA. The asterisks denote statistically significant differences after Student t test between the untreated control group and the EE₂ treated group at each time point. * p < 0.05 and ** p < 0.01. ns, not significant.