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RT-PCR to detect the amount of tetQ mRNA in BT4107 under TET-induced and non-TET-induced conditions. Lanes 1 and 2, tetQ product amplified by RT-PCR within the no-TET cells; lane 3, control to which RT was not added; lanes 4 and 5, tetQ product amplified by RT-PCR from the TET-treated BT4107 cells; lane 6, control to which RT was not added; lane 7, 1-kb standard. The arrow indicates the size of the amplified tetQ product (574 bp).
