Figure 2.

Survival analysis of the representative compounds 1 and Xyl-B in juglone-treated C. elegans using the Kaplan–Meier method. The worms were synchronized using sodium hypochlorite and sodium hydroxide. Forty-eight h later, the synchronized worms were further treated with 100 µM of the test compound in their food for an additional 48 h. All worms were then treated with 200 ng/mL of juglone, and the number of dead worms was immediately counted. DMSO was used as a control. The survival curve shows the time-dependent cumulative proportional survival rate. Three independent experiments were performed for each group, and each group contained 50 worms.