TABLE 2.
Transformation rates of H. pylori wild-type and NucT-deficient strains
| H. pylori strain | Transformation ratesa
|
||||
|---|---|---|---|---|---|
| Chromosomal DNA (gentamicin resistant)b | pILL444 (gentamicin resistant) | Chromosomal DNA (streptomycin resistant)b | Chromosomal DNA (kanamycin resistant)b | pHEL3 (kanamycin resistant) | |
| N6 | 5.6 × 10−6 | 1.23 × 10−6 | NDc | ||
| N6 nucT | 1.3 × 10−9 | <1 × 10−9 | ND | ||
| X47-2AL | ND | ND | 1.19 × 10−7 | 5.75 × 10−7 | 5.26 × 10−9 |
| X47-2ALNucT-Km | ND | ND | 6.70 × 10−9 | ||
| X47-2ALNucT-Gm | ND | 2.29 × 10−8 | 4.67 × 10−10 | ||
a
The transformation rates are expressed as the number of transformants per microgram of DNA per viable cell.
b
Chromosomal DNA from streptomycin-, gentamicin-, and kanamycin-resistant strains were used in the transformation experiments.
c
ND, not done.