Fig. 1. Thickness changes in gastrointestinal mucosa and muscle layers in VPA animal model of ASD. A. Thickness of mucosa and muscle was measured in the stomach, duodenum, jejunum, ileum and colon by analySIS TS AUTO software. Thickness of mucosa and muscle were measured from luminal surface to muscularis mucosa and from luminal surface to external muscle layer each. Thickness was measured in total six areas per sample with fixed interval (*p<0.05, ** p< 0.01. N = 4) B. Stomach and ileum section was stained by H&E. Picture of stomach and ileum was taken with 40x and 100x resolution, respecitively using 2048x1536-pixel digital CCD camera (DP70, Olympus, Tokyo, Japan) (TMu: Tunica Mucosa, TMs: Tunica Muscularis, im: Tunica Musccularis interna, em: Tunica Muscularis externa).
