Figure 2.

The inhibitory efficacy of PEE on UBUC cells. (A) Human T24 or J82 cells were incubated with increasing concentration of PEE for 72 h and cell viability was measured by MTT assay as described in Methods. (B) Time-dependent response experiments were underwent by treating human T24 or J82 cells with 50 μg/ml of PEE for various time durations. (C) The selectivity of PEE was determined by dose-response against E7 cell carried out by exposing the cells in the presence of the indicated concentrations for 72 h. All the diagrams in Figure  2 were the typical result of three independent experiments. All the data were expressed as mean ± standard deviation (S.D.) of the mean of four wells performed in triplicate. ** and *** denoted P < 0.01 and P < 0.001 respectively as compared to untreated cell using Student’s t-test.