FIG. 4.
Protein-protein interactions of MinCEc mutant proteins. The graph shows the interactions of wild-type (WT) and mutant E. coli MinC proteins (G135D, G154D, G161S, G171E, and P141A) with themselves (open bars) and with MinDEc (grey bars). An unpaired t test analysis showed that the differences in the strengths of self-interaction between wild-type MinCEc and the G135D, G154D, and P141A mutants were not significant (P = 0.128, P = 0.07, and P = 0.153, respectively). However, the difference in the strength of the self-interaction between wild-type MinCEc and the G171E mutant was significant (P = 0.005). The difference in the strength of the interaction between wild-type MinCEc or MinCEc G161S and MinDEc was significant (P = 0.005), whereas the interaction between the MinCEc P141A mutant and MinDEc was not significantly different (P = 0.091). The error bars indicate standard errors.