Table 2.
Description of primers used in PCR for the detection of virulence markers and erythromycin/clindamycin-resistance genes
| Target gene a | Sequence of the primer (5′ → 3′) | Amplicon size (bp) | Accession number b |
|---|---|---|---|
|
hylB |
F: TGTCTCCGAGGTGACACTTGAACT |
124 |
U15050.1/Y15903.1 |
| R: TTGTGTTGTGACGGGTTGTGGATG | |||
|
cylE |
F: TCGGAACAAGTAAAGAGGGTTCGG |
130 |
AF093787.2/AF157015.2 |
| R: GGGTTTCCACAGTTGCTTGAATGT | |||
| PI-1 |
F: AACCACTAGCAGGCGTTGTCTTTG |
147 |
EU929540.1/EU929469.1 |
| R: TGAGCCCGGAAATTCTGATATGCC | |||
| PI-2a |
F: GCCGTTAGATGTTGTCTTCGTACT |
117 |
EU929374.1/EU929330.1 |
| R: TTTACTGCGGTCCCAAGAGCTTC | |||
| PI-2b |
F: AAGTCTTGACCAAGGATACGACGC |
152 |
EU929426.1/EU929391.1 |
| R: ATCGTGTTACTTGCCCTGCGTA | |||
|
ermA |
F: CCGGCAAGGAGAAGGTTATAATGA |
190 |
EU492925.1/EU492926.1 |
| R: GCATTCACCCGTTGACTCATTTCC | |||
|
ermB |
F: GCTCTTGCACACTCAAGTCTCGAT |
117 |
EF422365.1/DQ250996.1 |
| R: ACATCTGTGGTATGGCGGGTAAGT | |||
|
mefA/E |
F: GCGATGGTCTTGTCTATGGCTTCA |
225 |
DQ445273.1/DQ445269.1 |
| R: AGCTGTTCCAATGCTACGGAT |
ahylB, hyaluronate lyase; cylE, hemolysin/cytolysin (β-H/C); PI-1, PI-2a, PI-2b, pilus islands; ermA, ermB cross-resistance to macrolides-lincosamide-streptogramin B; mefA/E resistance only to 14- and 15-membered ring macrolides. bThe nucleotide sequences of Streptococcus agalactiae genes deposited in the GenBank/EMBL databases used for specific primer design.