TABLE 1.
Primer | Sequence (5′→3′)a | 5′ position in BAp genomeb | PCR | Refer- ence |
---|---|---|---|---|
Leucine general primers | ||||
leuA.du2 | (CGGATCCTGCAG)GAT GAT GTW GAA TTT TCW TGY GAR GAY GC | 3431 (pLeu) | ilPCR | 48 |
leuA.dl3 | (CGGATCCGTCGAC)AR ACT WGC TTG WAR WGC TTG TTC WCC ATC | 3099 (pLeu) | ilPCR | 48 |
leuD.du2 | (CCCATCCTGCAG)GGW TGT GGW TCW TCW AGA GAR CAT GC | 7400 (pLeu) | Regular PCR | 48 |
leuA-R2 | (GGAATTC)WG TAT AWC CWA CWG TAT CWG G | 3556 (pLeu) | Regular PCR | TSc |
BCp-specific primers | ||||
gnd-lo1 | CAA GCT CAA AGA GAT TAT TTT GGA GCT C | 112951 | ilPCR | TS |
gnd-up1 | CTG CTC TAA TGA TAC TAC CTG CAC G | 112711 | ilPCR | TS |
dcd-dF1 | WGT GAY AVA GAY ATH GAR TGG | 113245 | Regular PCR | TS |
dcd-dR1 | ATK CCA WCC WGG RTC DAT NCK RTG | 113632 | Regular PCR | TS |
dcd-CloR1 | TAA AGA AGA ACG ACC ATC TAA CCA TCC | 113574 | Regular PCR | TS |
leuC-CloR1 | TCC TCC TCT ACC TTG CCT GCC TTC | 7063 (pLeu) | Regular PCR | TS |
BTc-specific primers | ||||
TcleuC-Fi | (GGAATTCCTCTAGA)A ATA TGG CTA TTG AAA TGG GAG CTA AAT CAG | 6405 (pLeu) | ilPCR | TS |
TcleuB-Ri | (GGAATTCGCTGCAG)ATT TCA AAA GCA AAA TTC GCT ATC CTA C | 5201 (pLeu) | ilPCR | TS |
TcleuD-Ri | (GGAATTCGCTGCAG)CGG AAG CCA TAA TCT AAA ATA GCC CAA AC | 7427 (pLeu) | ilPCR | TS |
TcleuA-Fi | (GGAATTCCTCTAGA)G ATA CAA CAT TAA GAG ATG GTG AAC AAG C | 3056 (pLeu) | Regular PCR | TS |
TcleuAi-F2 | (GGAATTC)AA ATT TTC GAG AAA CTG TTG ATC TAG C | 3371 (pLeu) | Regular PCR | TS |
mrcB900d-UR2 | ATA TAA WAG WGC WCC TTT GAC CAT ACC WAC | 217163 | Regular PCR | TS |
mrcB310d-UR1 | ATC WGG WAA ATC AAA WGS ACG WCG | 216574 | Regular PCR | TS |
Sequences in parentheses correspond to restriction enzyme sites for cloning purposes.
Main chromosome or pLeu plasmid.
TS, this study.