TABLE 1.

Primers used to amplify the leucine cluster and flanking regions in this study

Primer	Sequence (5′→3′)a	5′ position in BAp genomeb	PCR	Refer- ence
Leucine general primers
leuA.du2	(CGGATCCTGCAG)GAT GAT GTW GAA TTT TCW TGY GAR GAY GC	3431 (pLeu)	ilPCR	48
leuA.dl3	(CGGATCCGTCGAC)AR ACT WGC TTG WAR WGC TTG TTC WCC ATC	3099 (pLeu)	ilPCR	48
leuD.du2	(CCCATCCTGCAG)GGW TGT GGW TCW TCW AGA GAR CAT GC	7400 (pLeu)	Regular PCR	48
leuA-R2	(GGAATTC)WG TAT AWC CWA CWG TAT CWG G	3556 (pLeu)	Regular PCR	TSc
BCp-specific primers
gnd-lo1	CAA GCT CAA AGA GAT TAT TTT GGA GCT C	112951	ilPCR	TS
gnd-up1	CTG CTC TAA TGA TAC TAC CTG CAC G	112711	ilPCR	TS
dcd-dF1	WGT GAY AVA GAY ATH GAR TGG	113245	Regular PCR	TS
dcd-dR1	ATK CCA WCC WGG RTC DAT NCK RTG	113632	Regular PCR	TS
dcd-CloR1	TAA AGA AGA ACG ACC ATC TAA CCA TCC	113574	Regular PCR	TS
leuC-CloR1	TCC TCC TCT ACC TTG CCT GCC TTC	7063 (pLeu)	Regular PCR	TS
BTc-specific primers
TcleuC-Fi	(GGAATTCCTCTAGA)A ATA TGG CTA TTG AAA TGG GAG CTA AAT CAG	6405 (pLeu)	ilPCR	TS
TcleuB-Ri	(GGAATTCGCTGCAG)ATT TCA AAA GCA AAA TTC GCT ATC CTA C	5201 (pLeu)	ilPCR	TS
TcleuD-Ri	(GGAATTCGCTGCAG)CGG AAG CCA TAA TCT AAA ATA GCC CAA AC	7427 (pLeu)	ilPCR	TS
TcleuA-Fi	(GGAATTCCTCTAGA)G ATA CAA CAT TAA GAG ATG GTG AAC AAG C	3056 (pLeu)	Regular PCR	TS
TcleuAi-F2	(GGAATTC)AA ATT TTC GAG AAA CTG TTG ATC TAG C	3371 (pLeu)	Regular PCR	TS
mrcB900d-UR2	ATA TAA WAG WGC WCC TTT GAC CAT ACC WAC	217163	Regular PCR	TS
mrcB310d-UR1	ATC WGG WAA ATC AAA WGS ACG WCG	216574	Regular PCR	TS

^aSequences in parentheses correspond to restriction enzyme sites for cloning purposes.

^bMain chromosome or pLeu plasmid.

^cTS, this study.