Figure 2.

Structure of pARK-C plasmid series used for expression of gvpC fragments in Halobacterium sp. NRC-1 and ∆ gvp C strains. Location and transcriptional orientation of bla (pink), β-lactamase, for ampicillin resistance; mev (black), HMG-CoA reductase for mevinolin resistance; C1-C4, gvpC segments (blue), and rep (green), the Halobacterium pGRB replicase gene are shown. Position of the temperature-induced cspD2 promoter (labeled P_cs →), His-tag (red box), and KpnI, NdeI, AflII, AvrII, and BamHI restriction sites are indicated.