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. 2013 Dec 21;13:112. doi: 10.1186/1472-6750-13-112

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Copyright © 2013 DasSarma et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blotting analysis of Halobacterium sp. NRC-1∆ura3∆gvpC and derivative strains containing gvpC expression plasmids. Cell lysates (lanes 1–5) or gas vesicles (lanes 6–10) were electrophoresed on a 12% polyacrylamide-SDS gel, transferred to PVDF membrane, and probed with His-tag antibody followed by secondary antibody-alkaline phosphatase conjugate. Lanes 1 & 6: Halobacterium sp. NRC-1Δura3ΔgvpC, Lanes 2 & 7: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C1), Lanes 3 & 8: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C2), Lanes 4 & 9: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C3), Lanes 5 & 10: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C4).