gvpCdel5F
|
CGCAAGCTTATTACTTCTCTCCAGTCGATG
|
gvpC deletion construction
|
gvpCdel5R
|
GCGGGCAGTACTCATCTCGTCCTCGAGGCGTTTGTCTGTGACACTCAT
|
gvpCdel3F
|
GACGAGATGAGTACTGCCCGCCGGCCGGATGATAAAACATGA
|
gvpCdel3R
|
CGCAAGCTTACTCGTTGTAGACCAGCGTTG
|
gvpA-NdeI
|
CTCAAGGTATACCACTAGACCCTAAT
|
Amplification of gvpA promoter
|
gvpA-KpnI
|
ACTCATGGTACCTACTTCTCTCCAGT
|
gvpC-F1AflIIGvpC-F
|
GGTGTGCTTAAGATGAGTGTCACAGACAAA
|
gvpC gene segment amplification
|
gvpC-C1RAvrIIGvpC
|
CAGCCTAGGGTGGGTTGAGTTCATCTCTGT
|
gvpC-C2RAvrIIGvpC
|
CTGCCTAGGCGCGGTAGCGTCGAAGCTGTC
|
gvpC-C3RAvrIIGvpC
|
GTGCCTAGGTTCTGCTTCCGCTTCGAC
|
gvpC-C4RAvrIIGvpC
|
AGACCTAGGTGTTTTATCATCCGGCCG
|
gvpC-His-adapter F
|
CGTCTCCATATGCACCACCACCACCACCACCTTAAGCGTCTACCTAGGAGCGCTTGAGGATCCATC
|
His-tag adapter for gvpC and antigen fusion expression plasmid construction
|
gvpC-His-adapter R
|
GATGGATCCTCAAGCGCTCCTAGGTAGACGCTTAAGGTGGTGGTGGTGGTGGTGCATATGGAGACG
|
pKJ-cspD2F
|
GCTGGACTGCCTTTTCTTCG
|
Sequencing of promoters and inserts in pARK and pDRK series plasmids
|
pKJ-BamH1-3′160R
|
GTTACTCCACCGTCATTCAG
|
Universal F 20mer
|
GTTGTAAAACGACGGCCAGT
|
Sequencing across gvpC deletion
|
Universal R 20mer
|
CACAGGAAACAGCTATGACC
|
Luci Int R |
GTGGCTGAGGCAGATGAGGC |
Sequencing and determination of luciferase gene orientation |