Table 2.
Oligonucleotides used in this study
| Oligonucleotide | 5′-3′ sequence | Use |
|---|---|---|
|
gvpCdel5F |
CGCAAGCTTATTACTTCTCTCCAGTCGATG |
gvpC deletion construction |
|
gvpCdel5R |
GCGGGCAGTACTCATCTCGTCCTCGAGGCGTTTGTCTGTGACACTCAT |
|
|
gvpCdel3F |
GACGAGATGAGTACTGCCCGCCGGCCGGATGATAAAACATGA |
|
|
gvpCdel3R |
CGCAAGCTTACTCGTTGTAGACCAGCGTTG |
|
|
gvpA-NdeI |
CTCAAGGTATACCACTAGACCCTAAT |
Amplification of gvpA promoter |
|
gvpA-KpnI |
ACTCATGGTACCTACTTCTCTCCAGT |
|
|
gvpC-F1AflIIGvpC-F |
GGTGTGCTTAAGATGAGTGTCACAGACAAA |
gvpC gene segment amplification |
|
gvpC-C1RAvrIIGvpC |
CAGCCTAGGGTGGGTTGAGTTCATCTCTGT |
|
|
gvpC-C2RAvrIIGvpC |
CTGCCTAGGCGCGGTAGCGTCGAAGCTGTC |
|
|
gvpC-C3RAvrIIGvpC |
GTGCCTAGGTTCTGCTTCCGCTTCGAC |
|
|
gvpC-C4RAvrIIGvpC |
AGACCTAGGTGTTTTATCATCCGGCCG |
|
|
gvpC-His-adapter F |
CGTCTCCATATGCACCACCACCACCACCACCTTAAGCGTCTACCTAGGAGCGCTTGAGGATCCATC |
His-tag adapter for gvpC and antigen fusion expression plasmid construction |
|
gvpC-His-adapter R |
GATGGATCCTCAAGCGCTCCTAGGTAGACGCTTAAGGTGGTGGTGGTGGTGGTGCATATGGAGACG |
|
| pKJ-cspD2F |
GCTGGACTGCCTTTTCTTCG |
Sequencing of promoters and inserts in pARK and pDRK series plasmids |
| pKJ-BamH1-3′160R |
GTTACTCCACCGTCATTCAG |
|
| Universal F 20mer |
GTTGTAAAACGACGGCCAGT |
Sequencing across gvpC deletion |
| Universal R 20mer |
CACAGGAAACAGCTATGACC |
|
| Luci Int R | GTGGCTGAGGCAGATGAGGC | Sequencing and determination of luciferase gene orientation |