TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant genotype | Construction or source |
|---|---|---|
| Bacillus strains | ||
| BSA46 | trpC2 SPβ ctc::lacZ | 4 |
| PY22 | trpC2 | 29 |
| XS352 | trpC2 aph3′ 5′′/sigB ΔrsbST SPβ ctc::lacZ | 29 |
| BSZ11 | trpC2 sigB ΔrsbST rsbX::pWH25 SPβ ctc::lacZ | pWH25 → XS352 → BSA46 |
| BSA661-T0 | trpC2 aph3′5′′/sigB rsbT SPβ ctc::lacZ | pDRNT → XS352 |
| BSA661-T1 | trpC2 aph3′5′′/sigB rsbT1 SPβ ctc::lacZ | pT1 → XS352 |
| BSA661-T2 | trpC2 aph3′5′′/sigB rsbT2 SPβ ctc::lacZ | pT2 → XS352 |
| BSA661-T3 | trpC2 aph3′5′′/sigB rsbT3 SPβ ctc::lacZ | pT3 → XS352 |
| BSA661-T4 | trpC2 aph3′5′′/sigB rsbT4 SPβ ctc::lacZ | pT4 → XS352 |
| BSA661-T5 | trpC2 aph3′5′′/sigB rsbT5 SPβ ctc::lacZ | pT5 → XS352 |
| BSA661-T6 | trpC2 aph3′5′′/sigB rsbT6 SPβ ctc::lacZ | pT6 → XS352 |
| BSA661-T7 | trpC2 aph3′5′′/sigB rsbT7 SPβ ctc::lacZ | pT7 → XS352 |
| BSA661-T8 | trpC2 aph3′5′′/sigB rsbT8 SPβ ctc::lacZ | pT8 → XS352 |
| BSA661-T9 | trpC2 aph3′5′′/sigB rsbT9 SPβ ctc::lacZ | pT9 → XS352 |
| BSA661-T10 | trpC2 aph3′5′′/sigB rsbT10 SPβ ctc::lacZ | pT10 → XS352 |
| BSA661-T11 | trpC2 aph3′5′′/sigB rsbT11 SPβ ctc::lacZ | pT11 → XS352 |
| BSA661-T12 | trpC2 aph3′5′′/sigB rsbT12 SPβ ctc::lacZ | pT12 → XS352 |
| BSA661-T13 | trpC2 aph3′5′′/sigB rsbT13 SPβ ctc::lacZ | pT13 → XS352 |
| BSA661-T14 | trpC2 aph3′5′′/sigB rsbT14 SPβ ctc::lacZ | pT14 → XS352 |
| BSA661-T15 | trpC2 aph3′5′′/sigB rsbT15 SPβ ctc::lacZ | pT15 → XS352 |
| BSA661-T16 | trpC2 aph3′5′′/sigB rsbT16 SPβ ctc::lacZ | pT16 → XS352 |
| BSA661-T17 | trpC2 aph3′5′′/sigB rsbT17 SPβ ctc::lacZ | pT17 → XS352 |
| BSA661-T18 | trpC2 aph3′5′′/sigB rsbT18 SPβ ctc::lacZ | pT18 → XS352 |
| BSW13B | trpC2 PSPACrsbT SPβ ctc::lacZ | pRW13 → BSA46 |
| BSW14 | trpC2 PSPACrsbT3 SPβ ctc::lacZ | pRW10 → BSA46 |
| BSW15 | trpC2 PSPACrsbT4 SPβ ctc::lacZ | pRW11 → BSA46 |
| BSW16 | trpC2 PSPACrsbT5 SPβ ctc::lacZ | pRW12 → BSA46 |
| BSW17 | trpC2 PSPACrsbT7 SPβ ctc::lacZ | pRW14 → BSA46 |
| BSW18 | trpC2 PSPACrsbT13 SPβ ctc::lacZ | pRW15 → BSA46 |
| BSW19 | trpC2 PSPACrsbT16 SPβ ctc::lacZ | pRW16 → BSA46 |
| BSW44 | trpC2 aph3′5′′/sigB PArsbRΔrsbS rsbT SPβ ctc::lacZ | pRW22 → XS352 |
| BSW45 | trpC2 aph3′5′′/sigB PArsbRΔrsbS rsbT5 SPβ ctc::lacZ | pRW23 → XS352 |
| BSW46 | trpC2 aph3′5′′/sigB PArsbRΔrsbS rsbT7 SPβ ctc::lacZ | pRW24 → XS352 |
| BSW47 | trpC2 aph3′5′′/sigB PArsbRΔrsbS rsbT16 SPβ ctc::lacZ | pRW25 → XS352 |
| BSW48 | trpC2 ΔrsbST PSPACrsbT3 SPβ ctc::lacZ | pRW10 → BSZ11 |
| BSW49 | trpC2 ΔrsbST PSPACrsbT4 SPβ ctc::lacZ | pRW11 → BSZ11 |
| BSW50 | trpC2 ΔrsbST PSPACrsbT5 SPβ ctc::lacZ | pRW12 → BSZ11 |
| BSW13A | trpC2 ΔrsbST PSPACrsbT SPβ ctc::lacZ | pRW13 → BSZ11 |
| BSW51 | trpC2 ΔrsbST PSPACrsbT7 SPβ ctc::lacZ | pRW14 → BSZ11 |
| BSW52 | trpC2 ΔrsbST PSPACrsbT13 SPβ ctc::lacZ | pRW15 → BSZ11 |
| BSW53 | trpC2 ΔrsbST PSPACrsbT16 SPβ ctc::lacZ | pRW16 → BSZ11 |
| BSW69 | trpC2 aph3′5′′/sigB PArsbRΔrsbS rsbT3 SPβ ctc::lacZ | pRW36 → XS352 |
| BSW70 | trpC2 aph3′5′′/sigB PArsbRΔrsbS rsbT4 SPβ ctc::lacZ | pRW37 → XS352 |
| BSW71 | trpC2 aph3′5′′/sigB PArsbRΔrsbS rsbT13 SPβ ctc::lacZ | pRW38 → XS352 |
| Plasmids | ||
| pACT-2 | Apr vector with AD of GAL4 | Clontech |
| pAS2-1 | Apr vector with BD of GAL4 | Clontech |
| pUV70 | Apr protein fusion of RsbS with AD of GAL4 | 34 |
| pUV134 | Apr protein fusion of RsbR with AD of GAL4 | 34 |
| pUV166 | Apr protein fusion of RsbT with AD of GAL4 | 34 |
| pUV187 | Apr protein fusion of RsbU with AD of GAL4 | 34 |
| pJMObg | Apr protein fusion of Obg with AD of GAL4 | 27 |
| pWH25 | Apr SpecrrsbX | 29 |
| pUK19 | Apr Kanr | 19 |
| pUK19-1.1 | Apr KanrspoIIB | This study |
| pDG148 | Apr KanrlacI PSPAC | P. Stagier |
| pRW10 | Apr KanrlacI PSPACrsbT3 | This study |
| pRW11 | Apr KanrlacI PSPACrsbT4 | This study |
| pRW12 | Apr KanrlacI PSPACrsbT5 | This study |
| pRW13 | Apr KanrlacI PSPACrsbT | This study |
| pRW14 | Apr KanrlacI PSPACrsbT7 | This study |
| pRW15 | Apr KanrlacI PSPACrsbT13 | This study |
| pRW16 | Apr KanrlacI PSPACrsbT16 | This study |
| pRW22 | Apr SpecrPArsbRΔrsbSrsbT | This study |
| pRW23 | Apr SpecrPArsbRΔrsbSrsbT5 | This study |
| pRW24 | Apr SpecrPArsbRΔrsbSrsbT7 | This study |
| pRW25 | Apr SpecrPArsbRΔrsbSrsbT16 | This study |
| pRW26 | Apr protein fusion of rsbT3 with BD of GAL4 | This study |
| pRW27 | Apr protein fusion of rsbT4 with BD of GAL4 | This study |
| pRW28 | Apr protein fusion of rsbT5 with BD of GAL4 | This study |
| pRW29 | Apr protein fusion of rsbT7 with BD of GAL4 | This study |
| pRW30 | Apr protein fusion of rsbT8 with BD of GAL4 | This study |
| pRW31 | Apr protein fusion of rsbT13 with BD of GAL4 | This study |
| pRW32 | Apr protein fusion of rsbT15 with BD of GAL4 | This study |
| pRW33 | Apr protein fusion of rsbT16 with BD of GAL4 | This study |
| pRW34 | Apr protein fusion of rsbT18 with BD of GAL4 | This study |
| pRW36 | Apr SpecrPArsbRΔrsbSrsbT3 | This study |
| pRW37 | Apr SpecrPArsbRΔrsbSrsbT4 | This study |
| pRW38 | Apr SpecrPArsbRΔrsbSrsbT13 | This study |
| pUS19 | Apr Specr | 5 |
| pUSD19-1 | Apr Specr NdeI removed | This study |
| pDRNT | Apr SpecrPArsbRST | This study |
| pT1 | Apr SpecrPArsbRST1 | This study |
| pT2 | Apr SpecrPArsbRST2 | This study |
| pT3 | Apr SpecrPArsbRST3 | This study |
| pT4 | Apr SpecrPArsbRST4 | This study |
| pT5 | Apr SpecrPArsbRST5 | This study |
| pT6 | Apr SpecrPArsbRST6 | This study |
| pT7 | Apr SpecrPArsbRST7 | This study |
| pT8 | Apr SpecrPArsbRST8 | This study |
| pT9 | Apr SpecrPArsbRST9 | This study |
| pT10 | Apr SpecrPArsbRST10 | This study |
| pT11 | Apr SpecrPArsbRST11 | This study |
| pT12 | Apr SpecrPArsbRST12 | This study |
| pT13 | Apr SpecrPArsbRST13 | This study |
| pT14 | Apr SpecrPArsbRST14 | This study |
| pT15 | Apr SpecrPArsbRST15 | This study |
| pT16 | Apr SpecrPArsbRST16 | This study |
| pT17 | Apr SpecrPArsbRST17 | This study |
| pT18 | Apr SpecrPArsbRST18 | This study |