TABLE 3.
σB activation after induced expression of rsbTa
| Genotype | Mean β-galactosidase activity (Miller units) ± SD
|
|
|---|---|---|
| Uninduced | Induced | |
| rsbT/PSPACrsbT | 7 ± 2 | 25 ± 2 |
| rsbT/PSPACrsbT3 | 5 ± 2 | 6 ± 2 |
| rsbT/PSPACrsbT4 | 3 ± 2 | 4 ± 3 |
| rsbT/PSPACrsbT5 | 5 ± 3 | 19 ± 2 |
| rsbT/PSPACrsbT7 | 4 ± 2 | 14 ± 2 |
| rsbT/PSPACrsbT13 | 6 ± 2 | 5 ± 2 |
| rsbT/PSPACrsbT16 | 7 ± 2 | 12 ± 2 |
| ΔrsbS-T/PSPACrsbT | 2 ± 2 | 19 ± 4 |
| ΔrsbS-T/PSPACrsbT3 | 3 ± 3 | 3 ± 2 |
| ΔrsbS-T/PSPACrsbT4 | 2 ± 2 | 2 ± 2 |
| ΔrsbS-T/PSPACrsbT5 | 1 ± 1 | 1 ± 1 |
| ΔrsbS-T/PSPACrsbT7 | 1 ± 1 | 1 ± 1 |
| ΔrsbS-T/PSPACrsbT13 | 1 ± 1 | 2 ± 2 |
| ΔrsbS-T/PSPACrsbT16 | 1 ± 1 | 1 ± 1 |
a
B. subtilis strains with a wild-type rsbT allele at the sigB operon (rsbT) or a rsbS-T deletion at that locus (ΔrsbS-T) and one of the indicated rsbT alleles under the control of an IPTG-inducible promoter (PSPAC) on a multicopy plasmid (Table 1) were grown in Luria broth with (induced) or without (uninduced) IPTG. Samples were taken 1 h after IPTG addition and then assayed for σB-dependent (ctc::lacZ) β-galactosidase activity. The values (Miller units) represent the average of three independent assays.