TABLE 4.

Activation of lacZ reporter gene by RsbT-Gal4 chimeras^a

AD fusion	Reporter gene activity (Miller units) with BD fusion:
	Vector (pAS2-1)	rsbT	rsbT3	rsbT4	rsbT5	rsbT7	rsbT13	rsbT16
Vector (pACT2)	W	0.19	W	W	0.11	0.75	W	W
rsbR	0.05	1.35	W	0.04	0.83	1.02	W	W
rsbS	0.07	50.14	W	0.05	0.14	1.27	W	W
rsbT	0.03	0.68	W	0.05	0.14	0.59	W	W
rsbU	0.05	0.50	W	W	0.55	2.29	W	W
obg	0.05	0.48	W	W	0.21	1.53	W	W

^aThe columns represent yeast clones carrying the Gal4 DNA-binding domain (BD) vector pAS2- 1 either without insert DNA (vector) or with one of the rsbT alleles depicted, translationally fused to the Gal4 DNA-binding domain. The rows represent the Gal4 activation domain (AD) vector pACT2 with or without translational fusions to the RsbT regulators that are listed. Data depict reporter gene (GAL1_UAS-GAL1_TATA-lacZ) activity in yeast cells cotransformed with both of the plasmids indicated by the rows. W, pairings in which the yeast clones failed to give a blue color after freeze-thawing and exposure to X-Gal. The numbers represent a measurement of lacZ activity in those clones that displayed a blue colony phenotype after exposure to X-Gal. The values represent the average of duplicate assays of three independent clones for each plasmid pair. Aside from a Gal4-dependent lacZ gene, the host yeast strain (Y190) also contains GAL1_UAS-HIS3_TATA-HIS3, allowing histidine prototrophy if an active GAL4 regulator is formed. All of the blue colonies (i.e., pairings represented by numbers), but none of the white colonies (W), could form colonies on media lacking histidine. X-Gal, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside.