Figure 1.

Mevalonate and geranylgeranyl pyrophosphate reversed fluvastatin- and simvastatin-mediated inhibition of proliferation. YT-INDY was incubated for up to 72 hours in the presence of fluvastatin (50 µM), simvastatin (50 µM) or the solvents in which the drugs were dissolved. The statins or vehicles were added at the start of the experiment. Some statin-treated flasks also received mevalonate (1 mM) or geranylgeranyl pyrophosphate (GGPP) (10 µM). Cell counts were performed on each flask at 24 hour intervals. Each experiment was performed three times and the data was analyzed for statistical significance by Student’s t-test. Statistical significance is denoted by asterisks. *: p < 0.05, ***: p < 0.001.