Fig. 4.

Effect of AgNP on ALT, AST, LDH, and NO levels measured in culture media of unexposed control cells and cells exposed to 0.03, 0.12, and 0.24 mg/l of AgNP. The values were obtained using their respective assay kits, corrected according to the volume of culture media, and further normalized by considering the total cell number at the end of each generation. Data are expressed as mean   SD of two duplicate experiments. *indicates a statistically significant difference compared to the control. Within each dose group, among generations one to four, the differences were not statistically significant. first exposure, second exposure, third exposure, fourth exposure and average.