Table 1.

Primer and probe sequences

RT-PCR	Forward primer (plasmo1)	Reverse primer (plasmo2)	Probes
Pfal	5′-CTAGGTGTTGGATG-3′	5′-TTATGAGAAATCAAAGTCTTTGGGTT-3′	5′-FAM-AGCAATCTAAAAGTCACCTCGAAAGATGACT-DQ-3′
Pova	5′-CGACTAGGTTTTGGATG-3′		5′-VIC-CGAAAGGAATTTTCTTATT-DQ-3′
Pviv	5′-GACTAGGCTTTGGATG-3′		5′-VIC-AGCAATCTAAGAATAAACTCCGAAGAGAAAATTCT-DQ-3′
Pmal	5′-GACTAGGTGTTGGATG-3′		5′-FAM-CTATCTAAAAGAAACACTCAT-DQ-3′
PCR/genotyping	Forward primer	Reverse primer
Pfcrt	5′-GTTCTTGTCTTGGTAAATGTGCTCA-3′	5′-CAATT TTGTTTAAAGTTCTTTTAGCAA-3′

Legend: Pfal: P. falciparum; Pova: P.ovale; Pviv: P. vivax; Pmal: P. malariae; DQ: Darkquencher.