FIG. 2.

Effects of nonpolar pga gene disruptions on crystal violet binding of biofilms grown in polystyrene microtiter wells (A and B) and adherence of cells to borosilicate coverslips (C). Panels A and C depict results in the MG1655 strain background. Strains are represented by bars in panels A and B as follows: 1, MG1655; 2, XWMGΔABCD (ΔpgaABCD); 3, XWMGΔA (ΔpgaA); 4, XWMGΔB (ΔpgaB); 5, XWMGΔC (ΔpgaC); and 6, XWD146 (pgaD146::cam). (B) Strain identities were the same as in panel A, except that the strains were csrA mutants. Cultures were grown in LB medium at 26°C for 24 h. Asterisks denote significant differences relative to the corresponding parental strain (P < 0.001 [Tukey multigroup analysis]).