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Fractionation of polysaccharide extracts by gel filtration FPLC. Extracts from strain TRXWEC (MG1655 csrA cpsE pgaC880) containing either pPGA372 (pgaABCD) (A) or pUC19 (B) were fractionated by using a Sephacryl S-200 (HiPrep 16/60) column. Fractions (1.6 ml) were analyzed for neutral-sugar (▴) and, after hydrolysis, for hexosamine (▪). The column void volume, as determined with 2-MDa blue dextran, is indicated by a horizontal line.
