TABLE 1.
Strains, plasmids, and bacteriophage used in this study
| Strain, plasmid, or phagea | Description or genotype | Source or reference |
|---|---|---|
| E. coli K-12 strains | ||
| MG1655 | F− λ− | Michael Cashel |
| TRMG1655 | MG1655 csrA::kan | 52 |
| CF7789 | MG1655 ΔlacI-Z (MluI) | Michael Cashel |
| TRCF7789 | CF7789 csrA::kan | 60 |
| DJ4 | TRMG1655 cpsE::Tn10 | 32 |
| DJ6 | TRMG1655 ΔfimB-H | 32 |
| DJ2 | TRMG1655 csgA2::Tn105 | 32 |
| DJ24 | TRMG1655 ΔmotB uvrC-279::Tn10 | 32 |
| DJ25 | DJ24 ΔfimB-H | 32 |
| TRFMXWA* | DJ25 pgaA672::cam | This study |
| TRFMXWB* | DJ25 pgaB510::cam | This study |
| TRFMXWC* | DJ25 pgaC880::cam | This study |
| TRFMXWD* | DJ25 pgaD146::cam | This study |
| XWA672 | MG1655 pgaA672::cam | This study |
| XWB510 | MG1655 pgaB510::cam | This study |
| XWC880 | MG1655 pgaC880::cam | This study |
| XWD146 | MG1655 pgaD146::cam | This study |
| TRXWA672 | TRMG1655 pgaA672::cam | This study |
| TRXWB510 | TRMG1655 pgaB510::cam | This study |
| TRXWC880 | TRMG1655 pgaC880::cam | This study |
| TRXWD146 | TRMG1655 pgaD146::cam | This study |
| TRXWEC | DJ4 pgaC880::cam | This study |
| XWMGΔA | MG1655 ΔpgaA | This study |
| XWMGΔB | MG1655 ΔpgaB | This study |
| XWMGΔC | MG1655 ΔpgaC | This study |
| XWMGΔABCD | MG1655 ΔpgaABCD | This study |
| TRXWMGΔA | TRMG1655 ΔpgaA | This study |
| TRXWMGΔB | TRMG1655 ΔpgaB | This study |
| TRXWMGΔC | TRMG1655 ΔpgaC | This study |
| TRXWMGΔABCD | TRMG1655 ΔpgaABCD | This study |
| Plasmids | ||
| pCR-XL-TOPO | Cloning vector | Invitrogen |
| pCRPGA37 | E. coli pga locus in pCR-XL-TOPO | This study |
| pCR2.1-TOPO | Cloning vector | Invitrogen |
| pUC19 | Cloning vector | 55 |
| pPGA372 | pgaABCD in pUC19 | This study |
| pCRS5 | pgaA gene in pCR2.1-TOPO | This study |
| pCRR12 | pgaB gene in pCR2.1-TOPO | This study |
| pCRP93 | pgaD gene in pCR2.1-TOPO | This study |
| pETQwt | pgaC gene in pET-Blue | This study |
| pET-Blue | Expression vector | Novagen |
| pKD46 | For arabinose induction of λ Red system | 16 |
| pKD3 | Contains the cat gene | 16 |
| pCP20 | For FLP-recombinase production | 16 |
| Bacteriophage | ||
| P1vir | Strictly lytic P1 | Carol Gross |
| λNK1324 | Contains mini-Tn10cam transposon | 36 |
a
Original transposon insertion mutants, as displayed in Fig. 1A, used in the present study are indicated by an asterisk.