FIG. 3.

Northern blot analysis. (A) Total RNA was extracted from various P. aeruginosa strains, including PAO1 and the mvaT (PAO-P47) and mvaU (PAO-P46) isogenic mutants, and loaded on a 1% agarose gel. The positions of the major bands corresponding to the 23S, 16S, and 5S rRNA are indicated on the left, whereas the molecular size standards are indicated on the right. (B) After electrophoresis the RNA was transferred onto nitrocellulose and hybridized with one of various probes, including cupA1, cupB, and cupC1 probes. Each experiment was independently repeated up to three times with no significant variation observed.