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Analyses of 4°C cold stressed-leaves from wild-type (Col-0) and RS5 mutants. A, Semi-quantitative PCR of RS5 transcript abundance in the leaves of Col-0, rs 5–1 and 5–2 mutant plants stressed for 24 h. The ACTIN2 gene (ACT2, At3g18780) was used as a constitutively expressed control. B, GolS activity in the leaves of Col-0, rs 5–1 and 5–2 plants stressed for 14 d. C, RS activity in the leaves of Col-0, rs 5–1 and 5–2 plants stressed for 14 d.
